One of the adhesive cells culturing difficulties, is the damage they bear when dislodgement from culture flask. These injuries often cause a lot of cells destruction and mortality. In this article two methods of dislodgment of adhesive cells from culture Basks have been briefly explained. The first method involves 0.0%-0.07%. trypsin solution containing 1 m mol of EDTA and the second one contains 5-10m mol EDTA solution having albumin 1mg/ml.In albumin method the less EDTA solution is concentrated, the more time is needed to collect the cells. In trypsinizing method, for the cells of which adhesive power is higher, or if under specific care cell adhesiveness rises, higher trypsin percentage should be used.

Herein, the protein corona formation on the spherical metal nanoparticles is studied to investigate the possible effects of silver nanoparticles (AgNPs) on the protein activity and conformation. The digestion capability of trypsin was monitored on the human serum albumin (HSA) at standard enzymatic hydrolysis conditions in the absence and presence of different concentrations of AgNPs. So, the ratio of enzyme: HSA, the duration and the temperature of nanoparticle treatment were evaluated. The activity of treated trypsin molecules, in the form of hard (HC) and soft corona (SC) was studied using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE), and nano liquid chromatography electrospray (ion trap) mass spectrometry (nano LC-ESI/MS, LC-ESI/MS). In addition, the characteristics of silver nanoparticles and the formation of HSA corona on the nanoparticle surface were monitored using ultra-violet/visible spectroscopy (UV-Vis), Dynamic light scattering (DLS) and fluorescence spectrophotometry. The results demonstrated that not only the corona formation but also the AgNPs/Trypsin interaction decreases the hydrolysis potency of trypsin. Furthermore, interaction of AgNPs/HSA could influence both nanoparticles and HSA molecule features, companied with fluorescence study, that the HSA secondary structure. Also, LC-ESI/MS data revealed the most affected HSA triptics have α-helix structure.

Curcumin, a polyphenol yellow orange pigment present in Indian spice “turmeric”, is a member of the ginger family (Zingiberaceae). The extract of turmeric was obtained by using soxhlet apparatus at 45-55 oC, using methanol as solvent and then curcumin was isolated from methanolic extract by column chromatography. In the present study, a series of four new derivatives of curcumin (3a-d) were synthesized by reacting curcumin (1) with different alkyl halides (2a-d) using DMF and lithium hydride. The structures of natural curcumin and its synthetic derivatives were confirmed with the help of their 1H-NMR spectra and all these were screened against acetylcholinesterase, butyrylcholinesterase, lipoxygenase and trypsin enzymes. Curcumin and its derivatives exhibited moderate inhibitory potential against all aforesaid enzymes.